Plasmid

Part:BBa_K5048056:Design

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


plasmid pBAD lasR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1456
    Illegal PstI site found at 1436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1456
    Illegal PstI site found at 1436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1456
    Illegal BglII site found at 1432
    Illegal BamHI site found at 1422
    Illegal XhoI site found at 1428
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1456
    Illegal PstI site found at 1436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1456
    Illegal PstI site found at 1436
    Illegal NgoMIV site found at 322
    Illegal AgeI site found at 519
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1194
    Illegal BsaI site found at 1635
    Illegal BsaI site found at 2702
    Illegal SapI.rc site found at 3784


Design Notes

We replace the T7 promoter with araBAD promoter to prevent the leakage of promoter



Source

complete synthesis

References